Abstract
Once a bait has been generated and validated for the membrane yeast two-hybrid (MYTH) assay, it can be used for either high-throughput screening to generate a detailed interaction map (interactome) or in low-throughput experiments to examine interactions with specific targets. Here we describe how to carry out high-throughput MYTH library screening of a validated bait generated using integrated or traditional MYTH. The principles herein can be easily adapted for use in a smaller-scale format if required. A typical MYTH library screen can be completed in ∼3-4 wk.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
