Myristyltrimethylammonium Bromide (MYTAB) as a Cationic Surface Agent to Inhibit Streptococcus mutans Grown over Dental Resins: An In Vitro Study.

Paola Andrea Mena Silva,Fernanda Visioli,Julia Nunes,Mary Anne Melo,Fabrício Mezzomo Collares,Isadora Martini Garcia,Vicente Castelo Branco Leitune

doi:10.3390/jfb11010009

Abstract

This in vitro study evaluated the effect of myristyltrimethylammonium bromide (MYTAB) on the physical, chemical, and biological properties of an experimental dental resin. The resin was formulated with dental dimetacrylate monomers and a photoinitiator/co-initiator system. MYTAB was added at 0.5 (G0.5%), 1 (G1%), and 2 (G2%) wt %, and one group remained without MYTAB and was used as the control (GCtrl). The resins were analyzed for the polymerization kinetics, degree of conversion, ultimate tensile strength (UTS), antibacterial activity against Streptococcus mutans, and cytotoxicity against human keratinocytes. Changes in the polymerization kinetics profiling were observed, and the degree of conversion ranged from 57.36% (±2.50%) for G2% to 61.88% (±1.91%) for G0.5%, without a statistically significant difference among groups (p > 0.05). The UTS values ranged from 32.85 (±6.08) MPa for G0.5% to 35.12 (±5.74) MPa for GCtrl (p > 0.05). MYTAB groups showed antibacterial activity against biofilm formation from 0.5 wt % (p < 0.05) and against planktonic bacteria from 1 wt % (p < 0.05). The higher the MYTAB concentration, the higher the cytotoxic effect, without differences between GCtrl e G0.5% (p > 0.05). In conclusion, the addition of 0.5 wt % of MYTAB did not alter the physical and chemical properties of the dental resin and provided antibacterial activity without cytotoxic effect.

Highlights

Dental caries-linked bacteria growing in biofilms play a pivotal role in the initial formation and development of carious lesions
The antibacterial approach for dental materials relies on biological interactions of the bacteria grown over the materials, and the contact with antibacterial agents presents in the surface or is released by the materials [10,11,12,13]
The present study aimed to evaluate the potential ability of myristyltrimethylammonium bromide (MYTAB) to impair bacterial reduction when incorporated into dental resin at increasing concentrations

Summary

Introduction

Dental caries-linked bacteria growing in biofilms play a pivotal role in the initial formation and development of carious lesions. The antibacterial approach for dental materials relies on biological interactions of the bacteria grown over the materials, and the contact with antibacterial agents presents in the surface or is released by the materials [10,11,12,13] In this context, the achievement of surfaces able to reduce caries-linked biofilm formation over dental tissues or restorative structures has gained the attention of dental biomaterial researchers. The current understanding of the dental caries disease process and the new advances in dental materials promote the preservation of dental structure and the application of minimally invasive techniques [14] Under this framework, dental resins are the first option for the replacement of dental hard tissues that were lost due to carious lesions [15,16]. Dental resins with low viscosity can be successfully used to seal the biting surfaces of teeth, decreasing the incidence of caries lesions [18]

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