Abstract
We previously reported that mutations occurred in the gene myosin5 were responsible for resistance to the fungicide phenamacril in Fusarium graminearum. Here, we determined whether there is a functional link between phenamacril resistance and the myosin proteins FaMyo2B and Famyo2 in Fusarium asiaticum, which is the major causal agent of Fusarium head blight in China. We found that FaMyo2B acts jointly with FaSmy1 to affect resistance to phenamacril in F. asiaticum. We also found that FaMyo2B disruption mutant and Famyo2 deletion mutant were defective in hyphal branching, conidiation, and sexual reproduction. ΔFamyo2 also had an enhanced sensitivity to cell wall damaging agents and an abnormal distribution of septa and nuclei. In addition, the FaMyo2B and Famyo2 mutants had reduced pathogenicity on wheat coleoptiles and flowering wheat heads. Taken together, these results reveal that FaMyo2B and Famyo2 are required for several F. asiaticum developmental processes and activities, which help us better understand the resistance mechanism and find the most effective approach to control FHB.
Highlights
IntroductionFusarium graminearum sensu lato (teleomorph Gibberella zeae (Schwein.) Petch) is the main pathogens of Fusarium head blight (FHB) of wheat and other small cereal grains, which can produce harmful mycotoxins in infected grain and threat animals and human [1,2,3,4]
Altogether 11 phylogenetic species were the causal agent of Fusarium head blight (FHB) disease and these species belong to the Fusarium graminearum Schwabe species complex of B-trichothecene toxin producers [5,6,7,8,9]
We recently reported that mutations occurred in the gene myosin5 were responsible for resistance to phenamacril in F. graminearum [18] and Zhang et al [19] found that JS399-19 inhibited ATPase activity of FgMyo1 motor domain and JS399-19 had a serious impact on localization of the wild-type FgMyo1 at the tips of germlings
Summary
Fusarium graminearum sensu lato (teleomorph Gibberella zeae (Schwein.) Petch) is the main pathogens of Fusarium head blight (FHB) of wheat and other small cereal grains, which can produce harmful mycotoxins in infected grain and threat animals and human [1,2,3,4]. Altogether 11 phylogenetic species were the causal agent of FHB disease and these species belong to the Fusarium graminearum Schwabe species complex of B-trichothecene toxin producers [5,6,7,8,9]. In China, since 1936 when FHB was first reported, FHB disease have become
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