Abstract
A silver reduced graphene oxide (Ag-rGO) nanocomposite paste was prepared for the assay of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in children's saliva. The paste was modified with a solution of myoglobin (Myb) to form the stochastic biosensor, to improve the sensitivity of the method. The Ag-rGO powder and Myb-Ag-rGO paste were morphologically and structurally characterized by SEM, TEM and XRD measurements. For the molecular recognition of the hormones, the biosensor based on Myb-Ag-rGO reached determination limits of 8.5 × 10-3UIL-1 (2.0 × 10-12g/mL) for luteinizing hormone and 1.4 × 10-2UIL-1 (1.0 × 10-12g/mL) for follicle-stimulating hormone. The determination of both hormones in saliva samples collected from children was done with high reliability. Graphical Abstract.
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