Abstract
Myoglobin has been suggested to be a potential inhibitor of endogenous muscle proteases as different as cathepsin B, cathepsin L, cathepsin H and calpains all being supposed to be important in post-mortem muscle. The present work aimed at verifying the ability of myoglobin and its prosthetic group, hemin, to inhibit a series of endopeptidases including papain, cathepsin B, trypsin, calpains as well as two activities of the 20S proteasome. The conclusion of the present work was that inhibition of proteolytic activities of endopeptidases by myoglobin is an artifact. This was based on the following evidences: (1) a similar extent of inhibition was observed for all proteases tested whether myoglobin or hemin were added before starting the reaction or after having stopped it; (2) a quenching of the probes fluorescence by myoglobin and hemin; (3) no inhibition of calpains were found when assayed with non labeled casein as substrate and the activity expressed as the increase in the absorbency at 280 nm of the TCA soluble protein fragments. 1 Abbreviations: AMC, 7-amino-4-methyl coumaryl-amide; Boc, t-butyloxycarbonyl; DMSO, dimethyl-sulfoxyde; EDTA, ethylene-diamine-tetraacetic acid (tetrasodium salt); FITC, fluoroscein isothiocyanate; NAD, nicotinamide adenine dinucleotide; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulphate; Suc, succinyl; TCA, trichloro-acetic acid; Z, benzyloxycarbonyl. 1
Published Version
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