Myogenic Heartbeat in the Primitive Crustacean Triops longicaudatus.

Abstract

Pacemaker mechanisms in the heart of the primitive crustacean Triops longicaudatus were examined electrophysiologically. The heart is tubular and the heart wall consists of a single layer of myocardial cells. No nerve cells were found in the heart, either with methylene blue vital staining or by light microscopy of serial sections. The heart beats rhythmically at a frequency of 120 to 240 beats/min, and each beat is associated with a slow membrane potential change in the heart muscle. The amplitude of the slow potential varies widely and no spikes appear on it. The heart muscle cells are electrically coupled with each other and generate synchronous slow potentials. No localized portion of the heart exhibited a frequency that always preceded the others. The muscle activity could be phase-shifted by injection of a single brief current pulse and could be entrained to a lower or higher frequency by repeated brief current pulses injected into the muscle cell. The frequency of muscle activity could be changed by the injection of DC current into the muscle cell, and the change in frequency was linearly related to the intensity of the current. When the intensity of hyperpolarizing DC current exceeded a certain value, the muscle activity disappeared abruptly, and the heart stopped beating completely. These results show clearly that the heartbeat of Triops is myogenic. The heart is diffusely myogenic and should be regarded as a single muscle oscillator.