Myofibrillar, Mitochondrial and Valvular Morphological Alterations in Cardiac Hypertrophy among Copper-Deficient Rats

Abstract

Histological aspects of Cu-deficient hypertrophied rat hearts were evaluated to determine causal factors. Twenty-four male weanling rats were fed either Cu-adequate (8.0 mg/kg diet) or Cu-deficient (0.4 mg/kg diet) diets until 9 or 11 wk of age. Copper-deficient rats had increased mitochondrial:myofibrillar ratios compared with Cu-adequate rats. Mitochondria were vacuolated. Cristae seemed fragmented and matrix seemed translucent and they tended to distort the myofibrils. Glycogen granules and lipid droplets were more frequently observed in Cu-deficient rats. When the Cu-deficient rats were examined separately, the larger hearts in the older rats had smaller mitochondrial:myofibrillar ratios, but had larger myofibrillar volume densities. Valves from Cu-deficient rats had less connective tissue and seemed fragmented in areas. In 9-wk-old Cu-deficient rats, there was a negative correlation between heart: body weight ratios and bicuspid valve scores, whereas 11-wk-old rats demonstrated the same relationship for tricuspid valves with myofibril volume density. These results suggest that there are two components contributing to cardiac hypertrophy in copper deficient rats: 1) an enlarged mitochondrial area and 2) myofibrillar enlargement. Hematocrit values did not seem to be related to cardiac hypertrophy.