Myofibril size variation along the length of extraocular muscle in rabbit and rat. II: Global layer

Abstract

Systematic variation of myofibril profile size was observed along the length of both singly innervated fibers (SIFs) and multiply innervated fibers (MIFs) of the global (inner) layer of extraocular muscle (EOM). These findings contrast with the assumption that global layer fibers of EOM are structurally uniform along their length. Muscle fibers were reconstructed in serial sections along the global layer of rabbit and rat EOM. Long fibers of the ‘Pale’ SIF (fast twitch glycolytic) and the MIF (tonic) populations were sampled by EM in both proximal and distal portions of the muscle. In rabbit, myofibril size of the Pale SIFs showed a proximal-to-distal increase of 28% whereas the MIFs showed a proximal-to-distal decrease of 10%. In rat, these two fiber populations showed analogous smaller changes. Measures of profile size included the mean intercept length and the mean shortest path from test points within the profile to the profile boundary. The possible effect of sarcomere length variation was controlled by normalizing the measures to a constant spacing of the myosin filament lattice.