Abstract
BackgroundThere is a growing interest in developing non-invasive imaging techniques permitting infarct size (IS) measurements in mice. The aim of this study was to validate the high-resolution rodent Linoview single photon emission computed tomography (SPECT) system for non-invasive measurements of IS in mice by using a novel algorithm independent of a normal database, in comparison with histology.MethodsEleven mice underwent a left coronary artery ligature. Seven days later, animals were imaged on the SPECT 2h30 after injection of 173 ± 27 MBq of Tc-99m-sestamibi. Mice were subsequently killed, and their hearts were excised for IS determination with triphenyltetrazolium chloride (TTC) staining. SPECT images were reconstructed using the expectation maximization maximum likelihood algorithm, and the IS was calculated using a novel algorithm applied on the 20-segment polar map provided by the commercially available QPS software (Cedars-Sinai Medical Center, CA, USA). This original method is attractive by the fact that it does not require the implementation of a normal perfusion database.ResultsReconstructed images allowed a clear delineation of the left ventricles borders in all mice. No significant difference was found between mean IS determined by SPECT and by TTC staining [37.9 ± 17.5% vs 35.6 ± 17.2%, respectively (P = 0.10)]. Linear regression analysis showed an excellent correlation between IS measured on the SPECT images and IS obtained with TTC staining (y = 0.95x + 0.03 (r = 0.97; P < 0.0001)), without bias, as demonstrated by the Bland-Altman plot.ConclusionOur results demonstrate the accuracy of the method for the measurement of myocardial IS in mice with the Linoview SPECT system.
Highlights
There is a growing interest in developing non-invasive imaging techniques permitting infarct size (IS) measurements in mice
Animal models of myocardial infarction are commonly used to investigate pathophysiological mechanisms and to evaluate strategies aimed at reducing infarct size [1]
In patients with coronary artery disease, accurate quantification of infarct size can be obtained using single photon computed emission tomography (SPECT) and Tc-99mlabelled perfusion tracers [4]. This method has been validated in large animal models of myocardial infarction and can be used to serially measure infarct size in the same individuals
Summary
There is a growing interest in developing non-invasive imaging techniques permitting infarct size (IS) measurements in mice. The aim of this study was to validate the high-resolution rodent Linoview single photon emission computed tomography (SPECT) system for non-invasive measurements of IS in mice by using a novel algorithm independent of a normal database, in comparison with histology. In patients with coronary artery disease, accurate quantification of infarct size can be obtained using single photon computed emission tomography (SPECT) and Tc-99mlabelled perfusion tracers [4]. This method has been validated in large animal models of myocardial infarction and can be used to serially measure infarct size in the same individuals. Earlier investigators have succeeded in imaging rodent heart with small animals SPECT or positron-emission tomography (PET) cameras [5,6,7,8,9,10,11] and reported reasonably good correlations between infarct size measured by these modalities and that obtained with histology [7,8,9,10,11]
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