Abstract
Abstract 1. The effects of arachidonate (AA) on myocardial and coronary function, and the ability to metabolize AA have been explored for the first time in the heart of rainbow trout, Oncorhynchus mykiss . 2. On the isolated and perfused working heart exogenous AA (10 −7 and 10 −5 M) elicits a significant reduction of cardiac output and power output while heart rate is unaffected. 3. The negative inotropic effect is abolished in presence of 10 −5 M indomethacin. At the same AA concentration a pronounced increase in coronary resistance (175% change from baseline values) is apparent which is reduced but not abolished in the presence of 10 −5 M indomethacin. 4. 14 C-arachidonate is metabolized by trout ventricle homogenate into PGs E 2 , F 2α , D 2 , and in lesser amount into TXB and 6-keto-PGF 1α . Ca-ionophore A23187 enhances the production of both PGE 2 and PGF 2α . The lipoxygenase products assayed as the hydroxy acids (HETEs) appear to be less actively synthesized than prostanoids.
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