Myoblast Cell Interaction with Polydopamine Coated Liposomes

Rebecca Van Der Westen,Almar Postma,Leticia Hosta-Rigau,Kenneth N Goldie,Brigitte Städler,Duncan S Sutherland,Fernando Albericio

doi:10.1007/s13758-011-0008-4

Abstract

Liposomes are widely used, from biosensing to drug delivery. Their coating with polymers for stability and functionalization purposes further broadens their set of relevant properties. Poly(dopamine) (PDA), a eumelanin-like material deposited via the "self"-oxidative polymerization of dopamine at mildly basic pH, has attracted considerable interest in the past few years due to its simplicity, flexibility yet fascinating properties. Herein, we characterize the coating of different types of liposomes with PDA depending on the presence of oleoyldopamine in the lipid bilayer and the dopamine hydrochloride concentration. Further, the interaction of these coated liposomes in comparison to their uncoated counterparts with myoblast cells is assessed. Their uptake/association efficiency with these cells is determined. Further, their dose-dependent cytotoxicity with and without entrapped hydrophobic cargo (thiocoraline) is characterized. Taken together, the reported results demonstrate the potential of PDA coated liposomes as a tool in biomedical applications.

Highlights

Liposomes are among the most prominent objects employed in bionanotechnology
OD was embedded within the membrane with the goal to facilitate the PDA deposition i.e. to serve as anchor since it will copolymerize with the dopamine/PDA
We report the coating of liposomes with PDA and assess the interaction of these assemblies with myoblast cells

Summary

Introduction

Liposomes are among the most prominent objects employed in bionanotechnology In addition to their application as drug delivery vehicles [1, 2], they are employed in biosensing as labels [3] or as carriers for membrane proteins [4]. Coating liposomes with poly(ethylene glycol) (PEG) using PEGylated lipids is the typical and to date the most successful way to increase the circulation time [10, 11] when liposomes are used in drug delivery Alternative coatings such as mucoadhesive polymers, e.g. chitosan, have been considered to improve the mucoadhesive properties [12] or modifications based on the sequential deposition of interacting polymers onto liposomes [13]. Improved properties in terms of stability and cargo retention have been observed in the latter case, the separation of the free polymer

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