Myeloperoxidase genetic polymorphisms and susceptibility to Kawasaki disease in Taiwanese children.

Abstract

The aim of this study was to investigate the myeloperoxidase (MPO) -463G>A polymorphism in Kawasaki disease (KD) patients, and the relationship between gene polymorphism and MPO levels. A total of 334 KD children and 492 sex-matched controls were assayed for polymorphism analysis. TaqMan assays were used for genotyping. MPO was measured in 37 KD patients and 42 febrile controls. A significant linear trend of KD risk was found to be related to the G/G genotype (plinear trend=0.032). The combined genotypes (G/A and A/A) of MPO -463G>A were associated with a significantly decreased KD risk compared to the G/G genotype [adjusted odds ratios (AOR)=0.71, 95% confidence interval (CI): 0.52-0.99, p=0.040]. In addition, KD patients with A allele were associated with a significantly decreased KD risk as compared to those with G allele (AOR=0.73, 95% CI: 0.54-0.98, p=0.033). MPO levels were significantly elevated in KD patients in preintravenous immunoglobulin (pre-IVIG) stage compared to febrile controls (p=0.002). KD patients in pre-IVIG stage had significantly higher MPO levels than febrile controls in terms of G/G genotype (p=0.003) and G allele (p<0.001). KD patients with A allele had significantly lower MPO levels than those with G allele in post-IVIG acute stage (p=0.042). However, there was no significant difference of individual MPO change for KD patients from pre- to post-IVIG stage in terms of genotypes (p=0.837) or alleles (p=0.631). Our results suggest that G allele of MPO -463G>A polymorphism is a potential genetic marker for KD risk in Taiwanese children.