Myelin subfractions isolated from mouse brain: analysis of the lipid composition at three developmental stages.

Abstract

Lipids were examined in whole myelin and 8 myelin subfractions isolated from mouse brain at 18-24, 44-48 and 80-90 days of age. Relative to protein, total lipid was lowest in whole myelin isolated from the oldest animals as well as from subfractions isolated at greater sucrose densities, thus partially accounting for the observed myelin subfraction distribution pattern which shifted during development and an average peak density between 0.55 and 0.65 M sucrose to one banding between 0.60 and 0.70 M sucrose. Whole myelin and each myelin subfraction isolated at one age contained nearly the same ratio of sterol and phospholipid to galactolipid; these ratios decreased uniformly during development suggesting enrichment with galactolipid in all myelin subfractions. Sulfatide, as percentage of total galactolipid, was relatively constant during development and appeared to be slightly enriched in the denser myelin subfractions. The findings suggest that regardless of the origin(s) of the subfractions, an age-related mechanism exists in the central nervous system which modified myelin lipid composition relatively uniformly.