MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum.

Xian-Qing Liu,Yan Wang,Yu-Guang Zhou,Jin-Shan Ran,Jie Jin,Ze-Qing Feng,Fei Wang,Yi-Ping Liu

doi:10.1155/2015/692973

Abstract

Myeloid differentiation primary response gene 88 (MYD88), a universal adapter protein, plays an important role in activating the nuclear factor-κB (NF-κB) and regulating the expression of proinflammatory genes like tumor necrosis factor (TNF) and interleukin-1 (IL-1), which were highly involved in Salmonella Pullorum infection. To detect the relationship between polymorphisms of the MyD88 gene and Salmonella Pullorum disease, we screened the coding region (CDS) of the MYD88 gene by DNA pool construction and sequencing based on case-control study. Eight single nucleotide polymorphisms (SNPs) in the sequenced fragment (5 exons), 7 known loci and one novel mutation named G4810372T (SNP8), were found in the fifth exon. In addition, we found 7 nonsynonymous substitutions. The allele frequency of only one SNP, g.4810191C > T (SNP1), was significantly different (P < 0.05) between case and control groups. The genotype frequencies of SNP1 (g.4810191C > T) and SNP3 (g.4810257G > T) were of significant difference between the case and the control groups (P < 0.05). Collectively, SNPs of the MyD88 gene were significantly associated with susceptibility to Salmonella Pullorum infection, which can be used as a disease-resistant marker in chicken. These results provided a theoretical basis for future research on chicken breeding by marker-assisted selection.

Highlights

Salmonella Pullorum is a typical bacterial disease that has threated the modern poultry industry over the past years
Host inflammatory reaction is mediated by the recognition of pattern-recognition receptors (PRRs), which was activated by pathogen-associated molecular patterns (PAMPs) [3]
The intersubject differences in immunity that associated with missense mutations in innate immune genes, such as major histocompatibility complex (MHC) [22, 23] and myxovirus resistance gene (Mx) [24], have significantly increased in chicken

Summary

Introduction

Salmonella Pullorum is a typical bacterial disease that has threated the modern poultry industry over the past years. As a universal adaptor protein, MyD88 plays an important role in activating the innate immune system [10]. MyD88 was reported to play a crucial role in defensing against pathogenic infection for its extremely differential expression between spleen and muscle [15, 16]. Though some studies broadly reveal the function of MyD88 in TLR signaling pathways [18], the relationship between polymorphisms of the MyD88 gene and Pullorum infection in chicken has not been reported. In view of the importance of the MyD88 gene, it is necessary to analyze its sequence variations and to study whether its polymorphisms are associated with intersubject differences against salmonella. We selected chickens infected and uninfected with Salmonella Pullorum to conduct an association analysis with MyD88, aiming to provide a theoretical reference for poultry markerassisted selection

Materials and Methods

Result

Findings

Discussion