Mycotoxins survey in feed materials and feedingstuffs in years 2015–2020

Abstract

Mycotoxins are secondary metabolites produced by several fungi, mainly from Penicillium, Fusarium, and Aspregillus genera. They can contaminate many raw materials both in the field and during storage under very different conditions. This study aimed to evaluate mycotoxin contamination levels of raw materials and products for animals in Poland in the years 2015–2020. Producers, farmers, and veterinary doctors delivered a total of 3980 samples (642 maize samples, 2027 feed samples, 990 small grain samples, 142 maize silage samples and 179 TMR samples). Samples were analyzed for the occurrence of several mycotoxins, including aflatoxins, fumonisins, ochratoxin A, deoxynivalenol, nivalenol, T-2 toxin, H-2 toxin, and zearalenone. Trichothecenes, zearalenone, and fumonisins were determined using the LC-MS/MS technique. Ochratoxin A and aflatoxins were determined using the HPLC-FLD method. Deoxynivalenol and zearalenone were the most common contamination in maize samples (97.3% and 98.4%, respectively) and feed samples (99.7% and 100% samples, respectively). They were also present in all maize silage and TMR samples. The highest concentration levels of deoxynivalenol and zearalenone were 16,889 μg/kg in the wheat sample and 1420 μg/kg in the maize sample. Additionally, in 51 samples, mycotoxins level (mainly deoxynivalenol and zearalenone levels) was above recommended levels set by the European Union. The present study showed that both feeding and raw materials are contaminated by mycotoxins, often by more than one.