Abstract
In order to investigated current occurrence of major mycotoxins in dietary kelp in Shandong Province in Northern China, a reliable, sensitive, and rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of the 7 most frequent mycotoxins, including 3-acetoxy deoxynivalenol (3AcDON), 15-acetoxy deoxynivalenol (15AcDON), Deoxynivalenol (DON), Fusarenon-X (F-X), Nivalenol (NIV), T-2 toxin (T-2), and Zearalenone (ZEA). Based on optimized pretreatment and chromatographic and mass spectrometry conditions, these target analytes could be monitored with mean recoveries from 72.59~107.34%, with intra–day RSD < 9.21%, inter–day RSD < 9.09%, LOD < 5.55 μg kg−1, and LOQ < 18.5 μg kg−1. Approximately 43 kelp samples were detected, 3AcDON/15AcDON ranged from 15.3 to 162.5 μg kg−1 with positive rate of 86% in Shandong Province in Northern China. Considering there were no related investigations about mycotoxin contamination in kelp, the high contamination rate of 3AcDON/15AcDON in kelp showed a neglected mycotoxin exposure pathway, which might lead to high dietary exposure risk to consumers.
Highlights
Mycotoxins are mainly produced by filamentous fungi in a complex matrix [1,2]
In order to investigate the dietary exposure of mycotoxins in kelp, 50 kelp samples were obtained from a local supermarket in Shandong Province, China
A sonication based quantitative and confirmatory LC-MS/MS procedure was developed for the determination of 7 major mycotoxins (3AcDON, 15-acetoxy deoxynivalenol (15AcDON), DON, F-X, NIV, T-2, and ZEA)
Summary
Mycotoxins are mainly produced by filamentous fungi in a complex matrix [1,2]. Mycotoxins can contaminate different agricultural commodities and they are mainly detected in cereals, such as barley, wheat, maize, and even fruit and related products [3,4,5,6]. Antibody-based immunoassays were applied for mycotoxin detection with advantages of simplicity, low–cost and high throughput These immunoassays mainly include enzyme linked immunosorbent assay (ELISA) [23,24,25], fluorescence polarization immunoassay (FPIA) [26,27,28], surface plasmon resonance (SPR) [29,30,31,32], flow cytometric microsphere immunoassay [33,34,35], and rapid strip tests [36,37].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
