Mycosynthesis of silver nanoparticles using marine fungi and their antimicrobial activity against pathogenic microorganisms

Abstract

ObjectivesAt the present time, there is a persistent need to get rid of environmental contaminants by eco-friendly, sustainable, and economical technologies. Uncontrolled disposal practices of domestic and industrial solid and liquid wastes led to water pollution which has negative impacts on public health, environment, and socio-economic development. Several water-borne diseases are spreading man to man by microorganisms such as pathogenic bacteria. For the protection of water bodies, all wastewater from various sources should be managed and remediated properly. Myco-remediation is a form of bioremediation in which fungi are used to get rid of contaminants. Fungi are attractive agents for the biosynthesis of nanoparticles especially silver nanoparticles (AgNPs) which are considered one of the most widely utilized nanoparticles because of their unique characteristics such as antibacterial, antiviral, antifungal, and anti-inflammatory properties. MethodsThis study uses silver nitrate and supernatants of four marine fungi; Penicillium simplicissimum, Aspergillus terreus, Aspergillus japonicus, and Aspergillus oryzae for extracellular biosynthesis of silver nanoparticles and to evaluate its activity against different pathogenic microorganisms. These nanoparticles may subsequently be applied for the treatment or nano-bioremediation of microbial contaminants in water bodies and improve water quality. ResultsSilver nanoparticles were synthesized and the results revealed that spherical and well-dispersed nanoparticles of different sizes were formed with sizes ranging between 3.8 and 23 nm. Characterization results approved the existence of stable nanocrystalline elemental silver. Antibacterial activity results revealed that AgNPs can be used as a powerful antimicrobial agent for several pathogenic bacteria, yeast, and fungi. Among the biosynthesized NPs mediated by the four marine fungi, AgNPs mediated by A. japonicus (5 mM) had the highest antibacterial activity, while AgNPs mediated by Penicillium simplicissmum (8 mM) had the highest antifungal activity. ConclusionMarine fungi can biosynthesize stable AgNPs that exhibit potent antimicrobial activity against a variety of pathogens.