Myco-synthesis of silver nanoparticles from Trichoderma harzianum and its impact on germination status of oil seed

Abstract

The present study highlighting on the biosynthesis of silver nanoparticles by using the fungus Trichoderma harzianum. The cell filtrate of Trichoderma harzianum reacted with 1 mM silver nitrate solution, resulting the formation of silver nanoparticles within 3 hours. The silver nanoparticles were characterized by Visual analysis, UV-Vis absorption spectroscopy and Transmission electron microscopy (TEM). Biosynthesized silver nanoparticles exhibited maximum absorbance at 440nm in UV-Vis spectroscopy. TEM showed polydisperse spherical and occasionally ellipsoid nanoparticles in the size range from 19-63 nm and average size 34.77 nm. Disease free healthy looking seeds of Sunflower (Helianthus annuus) and Soybean (Glycine max) per-soaked in 3 days old silver nanoparticles solution of Trichoderma harzianum for 2hr and 5hr soaking period and. It is clear form the result that percentage of seed germination was enhanced irrespective of the myco-synthesized silver nanoparticles solution. T. harzianum synthesized silver nanoparticles showed increase in percentage of seed germination with increased in soaking time of silver nanoparticles solution. T. harzianum synthesized silver nanoparticles observed optimistic effect on seed germination. Therefore biosynthesized silver nanoparticles have biological assay used in agricultural purposes to increases the viability of seeds.