Abstract
Purpose: To produce and characterize silver nanoparticles using Candida albicans and evaluate its antibacterial properties. Methods: Extracellular silver nanoparticles were biosynthesized using C. albicans . The biomass obtained from cultures of C. albicans was used to synthesize silver nanoparticles in 1.5 mM silver nitrate solution. Characterization of the biosynthesized nanoparticles was carried out using ultraviolet (UV)- visible spectrometry and scanning electron microscopy (SEM). The antibacterial properties of the nanoparticles were determined by agar disc diffusion method against Escherichia coli and Staphylococcus aureus . Results: Incubation of C. albicans with silver nitrate solution produced silver nanoparticles after 2 – 4 h as evidenced by change in the color of the reaction mixture. The formation of silver nanoparticles was confirmed by UV-visible spectroscopy which showed absorption peak between 300 – 800 nm, being the characteristic wavelength range of silver nanoparticles. SEM revealed the varying morphology of the nanoparticles which had a size range of 20 – 80 nm. Furthermore, the nanoparticles showed significant antimicrobial activity (p < 0.05). Conclusion: The biosynthesized silver nanoparticles hold some promise for various industrial applications, including drug delivery. Keywords: Antibacterial activity, Candida albicans , Escherichia coli , Mycosynthesis, Silver nanoparticles, Staphylococcus aureus
Highlights
The use of nanotechnology in medicine is set to spread rapidly
Silver nanoparticles synthesized from C. albicans were tested for antibacterial activity against pathogenic microorganisms E. coli ATCC 39403 and S. aureus ATCC 25923
Silver nanoparticles were synthesized from silver nitrate by C. albicans
Summary
The use of nanotechnology in medicine is set to spread rapidly. In the last decades, the treatment of bacterial infections has become increasingly complicated due to their resistance mechanisms. Silver nanoparticles have proved to be most effective metallic nanoparticles as they have potent antimicrobial efficacy against various pathogenic microorganisms including bacteria such as Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Leuconostoc mesenteroides, Bacillus subtilis, Klebsiella mobilis, and Klebsiella pneumonia. Silver nanoparticles synthesized from C. albicans were tested for antibacterial activity against pathogenic microorganisms E. coli ATCC 39403 and S. aureus ATCC 25923. The antibacterial activity of silver nanoparticles was tested by the agar disc diffusion method according to the Clinical and Laboratory Standards Institute protocol (M02 – A11) for bacteria with some slight modification. Both bacteria were cultured on Nutrient Agar (NA, Merck, Germany) and passaged twice to ensure viability and purity.
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