Abstract
This study describes for the first time the use of high-resolution nuclear magnetic resonance (NMR) on Klamath (Aphanizomenon flos-aquae, AFA) blue-green algae directly on powder suspension. These algae are considered to be a "superfood", due to their complete nutritional profile that has proved to have important therapeutic effects. The main advantage of NMR spectroscopy is that it permits the detection of a number of metabolites all at once. The Klamath alga metabolome was revealed to be quite complex, and the most peculiar phytochemicals that can be detected directly on algae by NMR are mycosporine-like amino acids (porphyra-334, P334; shinorine, Shi) and low molecular weight glycosides (glyceryl β-d-galactopyranoside, GalpG; glyceryl 6-amino-6-deoxy-α-d-glucopyranoside, ADG), all compounds with a high nutraceutical value. The presence of cis-3,4-DhLys was revealed for the first time. This molecule could be involved in the anticancer properties ascribed to AFA.
Highlights
Aphanizomenon flos-aquae (AFA) is a wild freshwater unicellular microalga that spontaneously grows in copious amounts in Upper Klamath Lake (Klamath Falls, OR, USA) a volcanic lake with hot, deep and mineral rich waters
We present, for the first time to the best of our knowledge, the application of highresolution nuclear magnetic resonance (NMR) spectroscopy to the study of deuterated water (D2O)
Angle Spinning (HR-MAS) which is an NMR technique that bridges the divide between high-resolution
Summary
Aphanizomenon flos-aquae (AFA) is a wild freshwater unicellular microalga that spontaneously grows in copious amounts in Upper Klamath Lake (Klamath Falls, OR, USA) a volcanic lake with hot, deep and mineral rich waters. The proton (shifted by 0.3 – 0.5 ppm due to the different solvents employed) and carbon chemical shifts (within 0-3 ppm) parallel those reported for the 6-amino-6-deoxy ring of ishigoside, a glyceroglycolipid isolated from the Brown Alga Ishige okamurae, with radical scavenging properties.[33] This seems to us another interesting finding, for it indicates the presence of glycerol 6-amino-6-deoxy glucosides in AFA.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
