Mycorrhizal function on soil aggregate stability in root zone and root-free hyphae zone of trifoliate orange

Abstract

Arbuscular mycorrhizal fungi (AMF) influence soil aggregate stability through their hyphae, roots, and glomalin-related soil protein (GRSP); however, the individual effect of these factors is difficult to distinguish. Pots separated by a 37-μm mesh bag buried in the middle of each pot was used to establish root zone (root + hyphae) and hyphae zone (roots free), where the Poncirus trifoliata seedlings were colonized by Funneliformis mosseae or Paraglomus occultum in root zone. AMF inoculation significantly increased shoot, root, and plant’s total biomass, soil organic carbon, GRSP fractions, 2–4 and 1–2 mm size water-stable aggregates, and mean weight diameter (MWD) in root or hyphae zone. Within root zone, root colonization and biomass presented stronger relationship with MWD than GRSP fractions. While, within hyphae zone, total of GRSP fraction was significantly correlated with MWD. The study, suggested further that root biomass and colonization were the main mechanisms in root zone for improving aggregate stability, whereas total of GRSP fractions was of paramount importance in hyphae zone. Mycorrhizal effect on aggregate stability was observed to be contrastingly different between root zone and hyphae zone.