Mycorrhizal communities of Vanilla planifolia in an introduction area (La Réunion) under varying cultivation practices

Abstract

Societal Impact StatementVanilla is one of the most valuable spices in the world. In Madagascar and La Réunion, the world's leading producers, vanilla is of great economic and cultural importance. Like all orchids, vanilla plants associate with mycorrhizal fungi in their roots forming mutualistic associations that allow them to grow and thrive. Understanding the diversity of mycorrhizal fungi adapted to vanilla cultivation, particularly in the Indian Ocean islands where they have never previously been studied, is becoming a necessity for maintaining vanilla crops in these regions in the face of climate change and the emergence of new pathogens.Summary The vanilla orchid (Vanilla spp.) is one of the most valuable cultivated plants worldwide. As with all orchids, vanillas form mycorrhizal associations with fungi in their roots, but their fungal partners have not been investigated outside their native geographic range in Central America. We investigated the whole fungal and mycorrhizal associations in cultivated vanilla (Vanilla planifolia) by sequencing the fungal ITS‐2 marker in the terrestrial and aerial roots using a metabarcoding approach. We selected plants cultivated in three conditions (i.e., cultivation under shade house, in openfield, or in the understory) in one locality of La Réunion island (Indian Ocean) and tested for a possible effect of cultivation practices on fungal communities. Cultivated vanillas in La Réunion mainly associate with Tulasnellaceae (75 OTUs) and Ceratobasidiaceae (8 OTUs). Among the seven most abundant Tulasnellaceae, six are similar to fungi detected in the roots of cultivated vanillas in Central America or in the roots of native orchids in La Réunion. Cultivation practices impacted both total fungal and mycorrhizal community compositions with no clear effect on fungal richness. Notably, Tulasnellaceae and Ceratobasidiaceae were scarce in aerial roots, except in the traditional cultivation in the forest understory. These results shed light on the geographical origins of mycorrhizal fungi of cultivated vanillas in La Réunion and show that they form a pool of both locally and globally distributed fungal partners. These mycorrhizal communities vary according to cultivation practices, and their potential roles in plant nutrition and resistance against pathogens await further attention.