Mycoplasma synoviae Based Molecular, Phylogenic and Pathological Studies in Chickens

Abstract

Background: Given that Mycoplasma synoviae (MS) has the potential to cause serious respiratory illness in chicken and cause significant financial loss to farmers, the present work was designed to investigate the MS-derived pathology along with detailed molecular study of the MS organism, isolated from natural cases of MS infection. Methods: The work was carried out in the Department of Veterinary Pathology, College of Veterinary Sciences and Animal Husbandry, Durg, Chhattisgarh, for the period of two years which was ended on October 2021. Total 428 birds were included in this study. Tissue samples from the suspected cases of MS infection were collected and stored at -20°C and 10% formalin for molecular analysis and histopathological study respectively. Tissues were collected from respiratory organs along with liver, kidney and spleen. Result: Overall prevalence of MS in the Chhattisgarh state was recorded as 6.30% under present investigation. Genome sequence of the PCR positive amplicon from MS confirmed case isolated from Chhattisgarh showed 99% homology with the other genome sequences available in NCBI website. Phylogenic analysis revealed highest similarities of Chhattisgarh isolate with the isolates of China and Iran that were imported from the GenBank. There were various degenerative, inflammatory and necrotic lesions was found in the respiratory system along with liver, kidney and spleen of MS positive birds.