Abstract
Analysis by an enzyme-linked immunosorbent assay for cytokines indicated that whole cells, intracellular materials and cell membranes of Mycoplasma salivarium induced interleukin-6 and interleukin-8 in a human gingival fibroblast cell line, Gin-1 cells. This was confirmed by reverse transcription-polymerase chain reaction analysis of mRNAs of these cytokines. Studies with inhibitors of second-messenger pathway indicated that a protein kinase C-dependent pathway was involved in the expression of the activity of the cell membranes. In addition, whole cells of other mycoplasmas ( M. hominis, M. arthritidis, M. arginini, M. fermentans, M. penetrans, M. pirum and M. pneumoniae) tested for comparative purposes were also shown to possess the activity. Thus, this study demonstrated that mycoplasmas possess the activity to induce interleukin-6 and interleukin-8 in human fibroblasts.
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