Abstract
Mycoplasma ovipneumoniae (M. ovipneumoniae) was isolated from nasal swabs obtained from sheep with respiratory manifestations in delta region, Egypt; wherein 31 sheep with different ages were suffer from nasal discharge, cough, pneumonia, keratoconjunctivitis in a flock of sheep containing 134 sheep. By mycoplasma culture, nine samples were positive from 31examined samples (29%). M.ovipneumoniae is detected in 33.33% (3 out of 9 isolates of mycoplasma) using PCR with specific primer. The high percentage of isolation found in more than one-year age group, strains were isolated from the nasal swabs and no detection from ocular swabs. Isolated M. ovipneumoni strain subjected to sequencing and was designated as NMD-EG016, which showed a 94.6% 16S-23S RNA intergenic spacer sequence identity with three USA strains (M. ovipneumoniae-2014-2278-10, Mycoplasma.sp. clone-10OR05 and M.ovipneumoniae-1992-6751-17) and >94.4with standard strain ATCC 29419.
Highlights
Mycoplasma species are recognized for many diseases as arthritis, respiratory, eye lesions, genital disease and mastitis (Sharif and Muhammad 2009), Mycoplasma Diseases leads to major economic losses in small ruminant (Nicholas, 2002)
Clinical examination Examined sheep showed, nasal and ocular discharge, ocular cloudiness and keratoconjunctivitis in addition to coughing and rise of body temperature, these signs were obvious in the age group less than 1 year old (16 months of age) Table (2)
M. ovipneumoniae considered the greatest mycoplasmas involved in sheep respiratory diseases
Summary
Mycoplasma species are recognized for many diseases as arthritis, respiratory, eye lesions, genital disease and mastitis (Sharif and Muhammad 2009), Mycoplasma Diseases leads to major economic losses in small ruminant (Nicholas, 2002). M. ovipneumoniae causes lethal pneumonia in sheep and goats as it is the infectious agent in ovine pleuropneumonia (Lin et al, 2008; Dassanayake et al, 2010). This organism is prevalent and highly contagious in almost every flock, causing major economic losses in the ovine industry worldwide. Compared to other pathogenic mycoplasmas, studies on M. ovipneumoniae are restricted by many aspects including the lack of the entire genomic sequence. This markedly hinders the understanding of the pathogenic mechanisms and the molecular basis of M. ovipneumoniae infection.
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