Mycoheterotrophic plants as indicators of post-agricultural forest regeneration: abundance of Hypopitys monotropa and Monotropa uniflora in post-agricultural forests changes through time

Abstract

Herbaceous layers in second-growth forests are shaped by past land use. Disturbances such as agriculture may impact populations of mycoheterotrophs, non-photosynthetic mycorrhizal plants that obtain carbon from fungal networks by altering mycorrhizal communities or removing trees they derive carbon from. I tested the hypotheses that two mycoheterotrophic forest herbs increase in abundance during succession and become most common in older forests as plant communities reassemble through time. Distributions of Hypopitys monotropa and Monotropa uniflora were sampled in Athens County, Ohio, USA. I surveyed populations in a 40-site post-agricultural forest chronosequence with five upland and five valley sites in each of four age classes: 40–60, 61–80, 81–100, and >130 years since canopy closure. Aspect and elevation were measured to assess environmental influence. Both H. monotropa and M. uniflora were most common in older stands with EM tree-rich canopy composition and west- or south-facing aspects, indicating influence of historical, biotic, and edaphic factors. Hypopitys was exclusive to forests >80 years old, while M. uniflora was present in younger stands. Abundance of both species was also significantly predicted by Fagaceae basal area. Because EM trees were also most abundant in south- and west-facing uplands, environmental influence appears to be mediated through canopy composition.