Mycobiont and Whole Thallus Cultures of Roccella Montagnei Bél. For the Biosynthesis of Secondary Compounds

Abstract

<p>The mycobiont and whole thallus cultures of Roccella montagnei Bel. were established using soredia as an inoculum.The mycobiont<br />cultures showed optimum growth, biomass and biosynthesis of compounds in Lilly and Barnett medium with glucose as a carbon source,<br />micronutrients and vitamins. After the incubation period of 180 days, the cultures were harvested, and their biomass and secondary<br />compound profiles were analysed. The HPTLC chromatogram of the acetone extract of the NT and mycobiont cultures revealed erythrin<br />as the major biosynthesized compound in both and identified as a key biosynthate by R. montagnei. Further, the NT biosynthesized 5<br />additional compounds and the mycobiont cultures biosynthesized 6 additional compounds. The molecular identity of the cultured<br />mycobiont was confirmed using nuclear ribosomal Internal Transcribed Spacer (ITS) as well as the secondary chemistry. Lichen<br />compound erythrin was identified as a key biosynthate by the cultures.<br />Key word:-Roccella montagnei, Mycobiont, Whole thallus culture, Soredia, Erythrin.<br />Abbreviation: - NT, Natural thallus; MYC, Mycobiont culture; WTC, Whole thallus culture; UV, Ultra violet wave length; HPTLC,<br />High performance thin layer chromatography; ITS, Internal transcribed spacer.</p><p> </p><p><a id="pub-id::doi" href="http://dx.doi.org/10.21756/cba.v0i.11014">http://dx.doi.org/10.21756/cba.v0i.11014</a></p>