Abstract
Sclerotinia sclerotiorum (Lib.) de Bary (1884) is economically important pathogen of rapeseeds damaging up to 20% of plants in Slovakia. There is a suggestion that S. sclerotiorum reproduces predominantly asexually, thus genetically identical clonal populations are dominating in fields. Genetically distinct isolates can be distinguished by mycelial compatibility-incompatibility grouping system. The aim of the study was to determine genetic variability of S. sclerotiorum based on mycelial interaction within a single oilseed rape field in Slovakia. S. sclerotiorum isolates were obtained from flower petals of commercial rapeseeds field in Hul, Slovakia (2018) by transferring a single hyphal tip to the plates with potato dextrose agar (PDA). Two sets of 20 isolates were paired in all possible combination and cultivated on PDA amended by bromophenol blue. Mycelial interactions were scored after 7 days.Each of the 20 isolates in 1st set was unique and belonged to the different mycelial compatibility groups (MCGs). In the 2nd set, 18 MCGs was determined with one isolate and only 1 MCGs consisted of 2 isolates. No prevalent MCG was found in this study, suggesting that S. sclerotiorum population affecting the target rapeseed field consisted of a very diverse group of isolates. The high level of incompatible reactions found in this first study from Slovakia may indicate that S. sclerotiorum undergoes frequent outcrossing in rapeseed stands.
Highlights
In 2019, rapeseed (Brassica napus L.) was grown on an area of about 147,000.00 ha (11% of arable land area) in Slovakia (ŠÚ SR, 2020)
No prevalent mycelial compatibility groups (MCGs) was found in this study, suggesting that S. sclerotiorum population affecting the target rapeseed field consisted of a very diverse group of isolates
Since there is no information available on MCGs of the S. sclerotinia populations in rapeseed fields in Slovakia, the aim of the study was to determine genetic variability of S. sclerotiorum based on mycelial interaction within a single oilseed rape field
Summary
In 2019, rapeseed (Brassica napus L.) was grown on an area of about 147,000.00 ha (11% of arable land area) in Slovakia (ŠÚ SR, 2020). Previous studies have suggested that homothallic S. sclerotiorum reproduces predominantly asexually and in fields is dominated by clonal populations that are genetically identical (Kohli et al 1992). Distinct isolates can be distinguished by a quick and inexpensive macroscopic assay of the self-nonself recognition method followed by inclusion them into mycelial compatibility-incompatibility groups (Kohn et al, 1990). Different mycelial compatibility groups (MCGs) represent genetically distinct isolates (Kohn et al, 1991). Data of the Kohn et al (1991) demonstrate that the mycelial incompatibility in S. sclerotiorum occurs even within a local field population. Since there is no information available on MCGs of the S. sclerotinia populations in rapeseed fields in Slovakia, the aim of the study was to determine genetic variability of S. sclerotiorum based on mycelial interaction within a single oilseed rape field
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
