MYB transcription factor family in sweet cherry (Prunus avium L.): genome-wide investigation, evolution, structure, characterization and expression patterns

Xunju Liu,Jiyuan Wang,Muhmmad Salman Zahid,Irfan Ali Sabir,Songtao Jiu,Iftikhar Hussain Shah,Muhammad Abdullah,Muhammad Aamir Manzoor,Caixi Zhang

doi:10.1186/s12870-021-03374-y

Xunju Liu, Jiyuan Wang + Show 7 more

Open Access

https://doi.org/10.1186/s12870-021-03374-y

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Abstract

Back groundMYB Transcription factors (TFs) are most imperative and largest gene family in plants, which participate in development, metabolism, defense, differentiation and stress response. The MYB TFs has been studied in various plant species. However, comprehensive studies of MYB gene family in the sweet cherry (Prunus avium L.) are still unknown.ResultsIn the current study, a total of 69 MYB genes were investigated from sweet cherry genome and classified into 28 subfamilies (C1-C28 based on phylogenetic and structural analysis). Microcollinearity analysis revealed that dispersed duplication (DSD) events might play an important role in the MYB genes family expansion. Chromosomal localization, the synonymous (Ks) and nonsynonymous (Ka) analysis, molecular characteristics (pI, weight and length of amino acids) and subcellular localization were accomplished using several bioinformatics tools. Furthermore, the members of distinct subfamilies have diverse cis-acting regions, conserved motifs, and intron-exon architectures, indicating functional heterogeneity in the MYB family. Moreover, the transcriptomic data exposed that MYB genes might play vital role in bud dormancy. The quantitative real-time qRT-PCR was carried out and the expression pattern indicated that MYB genes significantly expressed in floral bud as compared to flower and fruit.ConclusionOur comprehensive findings provide supportive insights into the evolutions, expansion complexity and functionality of PavMYB genes. These PavMYB genes should be further investigated as they seem to be brilliant candidates for dormancy manipulation in sweet cherry.

Highlights

The transcription factors (TFs) are the proteins which regulates the rate of transcription of genetic information from DNA to messenger Ribonucleic acid (RNA) through linkage with a specific DNA sequence
V-MYB was identified as first MYB Transcription factors (TFs) in the avian myeloblastosis virus [4]. c-MYB-like TF is was the first plant MYB TF which was discovered in Zea mays and justified for anthocyanin biosynthesis, [5]
We discovered that PavMYB genes from the same subfamily having identical domain distributions and compositions elaborated that these subfamilies had a similar evolutionary history

Summary

Introduction

The transcription factors (TFs) (sequence-specific DNAbindings factor) are the proteins which regulates the rate of transcription of genetic information from DNA to messenger RNA through linkage with a specific DNA sequence. Sabir et al BMC Plant Biology (2022) 22:2 crucial regulators of gene transcription along with at a DNA-binding domain, nuclear localization signal transactivation domain and oligomerization site. One of the largest TFs families in the plant kingdom has been classified as the MYB gene family [3]. MYB proteins characterize the major transcription factor families in the plant kingdom. V-MYB was identified as first MYB TF in the avian myeloblastosis virus [4]. C-MYB-like TF is was the first plant MYB TF which was discovered in Zea mays and justified for anthocyanin biosynthesis, [5]. Three types MYBTFs (A-MYB, B-MYB and c-MYB) were later discovered in many slime molds, fungi, insects and vertebrates [6]

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