Abstract

Constitutive mutations in the galactose operator in E. coli arise with a frequency ten times smaller than in the regulator gene. The operator constitutive mutations do not arise as a consequence of mutagen treatment. Operator constitutive mutations do not revert to wildtype spontaneously or after mutagen treatment. It is concluded that operator constitutive mutations are multisite mutations, and that the operator region is considerably smaller than the regulator gene in the gal operon.

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