Mutations in the cytoplasmic domain of P0 reveal a role for PKC-mediated phosphorylation in adhesion and myelination.

Wenbo Xu,Janne Balsamo,Gang Xu,Jack Lilien,Michael Shy,John Kamholz,Lisa Elferink

doi:10.1083/jcb.200107114

Wenbo Xu, Janne Balsamo + Show 5 more

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https://doi.org/10.1083/jcb.200107114

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Abstract

Mutations in P0 (MPZ), the major myelin protein of the peripheral nervous system, cause the inherited demyelinating neuropathy Charcot-Marie-Tooth disease type 1B. P0 is a member of the immunoglobulin superfamily and functions as a homophilic adhesion molecule. We now show that point mutations in the cytoplasmic domain that modify a PKC target motif (RSTK) or an adjacent serine residue abolish P0 adhesion function and can cause peripheral neuropathy in humans. Consistent with these data, PKCα along with the PKC binding protein RACK1 are immunoprecipitated with wild-type P0, and inhibition of PKC activity abolishes P0-mediated adhesion. Point mutations in the RSTK target site that abolish adhesion do not alter the association of PKC with P0; however, deletion of a 14 amino acid region, which includes the RSTK motif, does abolish the association. Thus, the interaction of PKCα with the cytoplasmic domain of P0 is independent of specific target residues but is dependent on a nearby sequence. We conclude that PKC-mediated phosphorylation of specific residues within the cytoplasmic domain of P0 is necessary for P0-mediated adhesion, and alteration of this process can cause demyelinating neuropathy in humans.

Highlights

Myelin is a multilamellar structure that surrounds axons in both the central nervous system and the peripheral nervous system (PNS),* facilitating nerve conduction
A subterminal 13 amino acid sequence in the cytoplasmic domain of P0 is essential for adhesion
To define the regions of the cytoplasmic domain of P0 that are essential for homophilic adhesion, we stably transfected mouse L cells and HeLa cells with cDNAs encoding wildtype P0 or P0 deletion mutants lacking the COOH-terminal 13 (⌬13), 28 (⌬28), 43 (⌬43), or 59 amino acids (⌬59)

Summary

Introduction

Myelin is a multilamellar structure that surrounds axons in both the central nervous system and the peripheral nervous system (PNS),* facilitating nerve conduction. The absence of P0 (Giese et al, 1992; Martini et al, 1995) or overexpression of P0 (Wrabetz et al, 2000) results in hypomyelination and peripheral neuropathy. Mutations in the human P0 gene cause the demyelinating peripheral neuropathy Charcot-Marie-Tooth disease 1B, the more severe Dejerine-Sottas syndrome, and congenital hypomyelination, all associated with muscle weakness, atro-. *Abbreviations used in this paper: HPRT, hypoxanthine phosphoribosyl transferase; PNS, peripheral nervous system; PVDF, polyvinylene difluoride; RACK, receptor for activated C kinase; RT, reverse transcription.

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