Mutations associated with boscalid and pyraclostrobin resistance of Botrytis cinerea from vegetable fields in Turkey

Abstract

Botrytis cinerea Pers. is a polyphagous fungal pathogen that can cause significant damage in the field, warehouse, and greenhouse conditions. In Turkey, fungicides with site-specific modes containing the active ingredients boscalid and pyraclostrobin are used to control B. cinerea. In this study, it was aimed to determine the mutations associated with resistance to boscalid and pyraclostrobin active substances in B. cinerea isolates obtained from tomato, pepper, eggplant, and lettuce in Turkey. In the in vitro bioassay, a conidial germination test was performed. It was determined that 26% of the isolates used in the bioassay test were resistant. After that, mutations associated with resistance were investigated. Mutations associated with boscalid resistance were found in 18 isolates whose SdhB gene was sequenced. No mutations were detected in sensitive MH51 and Ant34 isolates. It has been determined that N230I and H272R mutations were found to be frequent in B. cinerea populations in Turkey. P225F mutation was detected only in the B4 isolate obtained from the pepper in Antalya. The mutations associated with boscalid resistance detected in this study are reported for the first time in Turkey. The G143A mutation associated with pyraclostrobin resistance was detected in all the isolates sequenced.