Mutation rate analysis via parent-progeny sequencing of the perennial peach. II. No evidence for recombination-associated mutation.

Long Wang,Laurence D Hurst,Sihai Yang,Chao Qin,Yanchun Zhang,Dacheng Tian

doi:10.1098/rspb.2016.1785

Long Wang, Laurence D Hurst + Show 4 more

Open Access

https://doi.org/10.1098/rspb.2016.1785

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Abstract

Mutation rates and recombination rates vary between species and between regions within a genome. What are the determinants of these forms of variation? Prior evidence has suggested that the recombination might be mutagenic with an excess of new mutations in the vicinity of recombination break points. As it is conjectured that domesticated taxa have higher recombination rates than wild ones, we expect domesticated taxa to have raised mutation rates. Here, we use parent–offspring sequencing in domesticated and wild peach to ask (i) whether recombination is mutagenic, and (ii) whether domesticated peach has a higher recombination rate than wild peach. We find no evidence that domesticated peach has an increased recombination rate, nor an increased mutation rate near recombination events. If recombination is mutagenic in this taxa, the effect is too weak to be detected by our analysis. While an absence of recombination-associated mutation might explain an absence of a recombination–heterozygozity correlation in peach, we caution against such an interpretation.

Highlights

Both mutation rates and recombination rates vary between species and between regions within a genome [1,2]
What are the determinants of these forms of variation? Prior evidence has suggested that the recombination might be mutagenic with an excess of new mutations in the vicinity of recombination break points
Employing the same data, we focus on the possibilities that recombination might be mutagenic [7,8] and whether the recombination rate of domesticated peach is higher than that of wild peach, there commonly being a suggestion that domestication is associated with raised recombination rates [9 –11]

Summary

Introduction

Both mutation rates and recombination rates vary between species and between regions within a genome [1,2]. Employing the same data, we focus on the possibilities that recombination might be mutagenic [7,8] and whether the recombination rate of domesticated peach is higher than that of wild peach, there commonly being a suggestion that domestication is associated with raised recombination rates [9 –11]. If both are true some variation between genomic regions and between strains in the mutation rate may be attributable to recombination-associated mutation. We ask whether domesticated peach has a higher recombination rate than a wild close relative and whether mutations occur more often near recombination break points

Material and methods

Results

All intraspecific groups mean COs

Findings

Discussion