Mutation of the p53 tumour suppressor gene occurs preferentially in the chromophobe type of renal cell tumour.

Abstract

Genomic DNA from 30 non-papillary and 20 chromophobe renal cell carcinomas (RCCs), 30 papillary renal cell tumours, and 20 renal oncocytomas was screened for the presence of mutations in exons 5-8 of the p53 tumour suppressor gene by polymerase chain reaction-single strand conformation polymorphism analysis and direct DNA sequencing. Mutations leading to an amino acid change were found only in 6 out 20 chromophobe RCCs. Microsatellite analysis of chromophobe RCCs revealed the loss of one allele at chromosome 17p in 14 out of 18 informative cases. No mutation of the p53 gene was found in five sarcomatous RCCs or in seven tumours of stage IV. This study shows that mutation of the p53 tumour suppressor gene does not correlate with the specific loss of DNA sequences at chromosome 17 in chromophobe RCCs, nor can it be used as a prognostic parameter for RCCs in general.