Abstract

Bacillus velezensis is a kind of beneficial bacteria that is widely used in agriculture industry. Bacillus velezensis was irradiated with corona discharge generated by a needle-array high-voltage electrode. The results showed an improvement of activity of Bacillus velezensis by the corona discharge treatment was confirmed at an optimum input energy. Mutation of the Bacillus velezensis by the corona discharge treatment was also confirmed through an rRNA sequence alignment analysis. The enzyme activity of the mutated bacteria was greatly improved, which was a positive effect that can meet the production demand.

Highlights

  • Bacillus velezensis is a Gram-positive bacterium with rod shape that can produce spores

  • In the discharge-treated bacteria, the morphology of many strains was very different from the control strain

  • It can be seen from the figure that enzyme activity of eight strains (No 5~12) of these bacteria greatly improved, indicating beneficial variation occurred in this treatment

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Summary

Introduction

Bacillus velezensis is a Gram-positive bacterium with rod shape that can produce spores. It is a kind of aerobic or facultative anaerobic bacteria, which can be widely used in air, water, human, animal intestines, vegetables and food [1,2]. Bacillus velezensis plays an increasingly important role in the application of microorganisms [3]. Bacillus velezensis can produce spores even in adverse environments. Spores can be converted to powder without death of bacteria, which has obvious advantages over other biocontrol bacteria [4]. Some kinds of Bacillus velezensis are gradually developed and utilized in the production of enzymes, antibiotics, pesticides, surfactants, biological agents, flavor enhancers and nutrition and health products [5]

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