Mutation in the beta3 subunit of Guanine nucleotide-binding protein (GNB3) gene is not associated with Type II diabetes mellitus risk: a case–control study of a North Indian population

Abstract

Context: Genetics play a major role in development and pathophysiology of Type 2 diabetes mellitus (T2DM).Objective: To asses the association of Guanine nucleotide-binding protein (GNB3) (C825T) gene's polymorphism with T2DM.Materials and methods: A case–control study including 400 North Indians was performed using Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) approach to analyze genetic polymorphism.Results: No significant difference was observed in genotype and allele frequencies of GNB3 gene on comparing cases with controls.Discussion: Our study is in agreement with studies on Polish, Japanese, Hispanic-American and Danish populations who observed no significant association between GNB3 (C825T) polymorphism and T2DM.Conclusion: GNB3 (C825T) polymorphism is not associated with T2DM.