Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas

Abstract

Background:Kinase module of Mediator complex (‘CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions.Methods:Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing.Results:None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13.Conclusions:Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas.