Abstract
SPONTANEOUS mutations affecting uredospore colour in cereal rusts have been observed by several investigators. The mode of inheritance of the mutant colours in the wheat stem rust fungus Puccinia graminis tritici E. and H. and oat stem rust fungus P. graminis avenae E. and H. has been shown to follow a simple Mendelian pattern1,2 and such mutants serve as particularly useful markers in investigations of the role of asexual recombination in this group of fungi. In an attempt to produce uredospore colour mutants in the oat stem rust organism for this purpose, the mutagenic effect of the alkylating agent ethyl methane sulphonate (EMS) was investigated following its success in this respect in such widely diverse organisms as bacteriophage4 and barley5.
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