Mutants at gliadin loci on the basis of the common wheat cultivar Bezostaya 1

Abstract

Aim. The aim of this study was to isolate and propagate mutants at gliadin loci developed on the basis of the common wheat cultivar Bezostaya 1. Methods. We searched for spontaneous and gamma-irradiation induced mutations at gliadin loci among the progeny of F1 and F2 plants from crosses between near-isogenic lines by gliadin loci on the basis of the cultivar Bezostaya 1, including lines with the wheat-rye 1BL.1RS translocation. To identify mutations, we performed acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins. Results. On the basis of the common wheat cultivar Bezostaya 1, five mutants (six mutations) at gliadin loci were isolated and propagated, four of which were described for the first time. Three mutations occurred at the Gli-R1 locus involved in the wheat-rye 1BL.1RS translocation (the loss of secalins, intensification of a secalin component, and increased mobility of a secalin component). Two mutations were identified in the allele Gli-B1b, one caused the null-allele at the Gli-A2 locus. Conclusions. The material of mutants is of importance for studying the role of certain groups of storage proteins and their components in quality determination, as well as mechanisms of regulation of storage protein synthesis.
 Keywords: Triticum aestivum, gliadin, secalin, mutation, 1BL.1RS translocation.