Abstract
Background: Nano composites are widely used in medical sciences recently. Identification of chemical mutagens is an important issue in drug safety. Objectives: The aim of this study was to investigate mutagenicity and antibacterial activity of various generations of poly (amid amine) (PAMAM) dendrimers and agonic acid poly (amid amine) (PAMAM) Nano composite G2 on enteric pathogenic bacteria. Materials and Methods: Disc diffusion method, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) determination were performed for antibacterial activity survey. Mutagenic properties were determined by Ames assay. Results: Various dilutions of PAMAM generations (G2, G3, G4 and G5) and agonic acid PAMAM Nano composite G2 had antibacterial effect on Escherichia coli, Salmonella enterica, Salmonella typhimurium, Enterococcus faecalis and Staphylococcus aureus. In Ames assay, reverted colonies were increased by PAMAM generations increasing. Moreover, reverted colonies Ames assay were lower when Nano composite G2 was applied. Conclusions: PAMAM generations and agonic acid PAMAM Nano composite G2 contain compounds with therapeutic potential and antibacterial characteristics, which can be used in medicine. Because some chemicals have mutagenic and carcinogenic properties, identification of these chemical is of great importance.
Highlights
Nano composites are widely used in medical sciences recently
Various generations of polies amid amine (PAMAM) dendrimers and agonic acid PAMAM Nano composite G2 on bacteria showed an antibacterial effect on E. coli, S. typhi, S. typhimurium, E. faecalis and S. aureus
For S. typhimurium and S. aureus, more effective MIC (0.001) of nanocomposite G2 was similar to G2 and G3 MIC and for E. faecalis, optimum MIC (0.0001) of Nano composite G2 was similar to G4 and G5 MIC
Summary
Nano composites are widely used in medical sciences recently. Identification of chemical mutagens is an important issue in drug safety. Objectives: The aim of this study was to investigate mutagenicity and antibacterial activity of various generations of poly (amid amine) (PAMAM) dendrimers and agonic acid poly (amid amine) (PAMAM) Nano composite G2 on enteric pathogenic bacteria. Conclusions: PAMAM generations and agonic acid PAMAM Nano composite G2 contain compounds with therapeutic potential and antibacterial characteristics, which can be used in medicine. Dendrimers are a class of hyper branched and Nano scale macromolecules All these materials are originated from a nucleus and described as three-dimensional macromolecules with branching structures. Salmonella typhimurium micro some assay (Ames test) is an accepted short-term bacterial assay or a biological test to detect mutagenic potential of chemical compounds and identifying substances that can produce genetic damage leading to gene mutation [14, 15]
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