Abstract
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.
Highlights
The Brazilian savanna, known as “cerrado”, is one of the most biologically diverse areas in Brazil
Plathymenia reticulata Benth, popularly known as “vinhático”, belongs to the Leguminosae family, which is a source of high quality wood
Della Torre A, et al Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth
Summary
The Brazilian savanna, known as “cerrado”, is one of the most biologically diverse areas in Brazil. Plathymenia reticulata Benth, popularly known as “vinhático” (wine-like), belongs to the Leguminosae family, which is a source of high quality wood. It may be useful in restoring deforested areas, mainly in the savanna region, making it an economically important tropical species [1]. P. reticulata extract showed in vitro antimicrobial activity against Streptococcus mutans and Staphylococcus sp. The recently described antiophidian property of P. reticulata hydroalcoholic extract obtained from its bark showed that tannins from this plant were able to precipitate proteins of both Crotalus and Bothrops venoms [8]. Della Torre A, et al Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth
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