Abstract

AbstractMutagenic effects of gamma rays and EMS using both single and combined dosages were examined in opium poppy (Papaver somniferum L.). For inducing both macro‐ and micro‐mutations in the present study, combined mutagenic doses, especially 5 kR + 0.4 % EMS appeared to be greatly superior to all single doses of the two mutagens: gamma rays and EMS. The lowest dose of combined mutagen (5 kR + 0.2 % EMS) was about twenty times more effective than the most effective dose (15 kR) of gamma rays and twice as effective as the most effective concentration (0.4 %) of EMS. The combined dose 5 kR + 0.4 % EMS enhanced morphine content (%) to 0.98 as against 0.64 in the control. In accordance with earlier findings (GAUL 1964) the study revealed a correlation between induced macro‐ and micro‐mutations.

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