Abstract
Mutant strains of GO112 and BM302 with a high 2-keto-L-gulonic acid(2KLG) transformation rate induced by ion beam implantation wereseparately and combinatorially compared with the original strainsGO29 and BM80 to study the mutagenic effects of ion beamimplantation. Both the sole GO112 and mixed BM302:GO112 demonstratedimproved SNDH activity and 2KLG yield compared to the originalstrains. The mutant combinations of BM302:GO112 showed a longerstationary phase and higher biomass than BM80:GO29. The mutant BM302exhibited a stronger capacity to maintain a stable pH environment atmixed fermentation with Gluconobacter oxydans (G. oxydans) for 2KLGtransformation and facilitated the growth of G. oxydans comparedwith the original strain BM80. The promotive capacity to L-sorbosonedehydrogenase (L-SNDH) from the supernate of BM302 was 1.6-foldhigher than that of BM80. Genes encoded SNDH in GO29 and GO112 wereamplified and sequenced, and mutations including three transitions(CG → TA, CG → TA, GC → AT) andone transversion (AT → TA) were confirmed from GO29 toGO112. The corresponding amino acid was changed as Leu →Phe, Arg → Gln and Asn → Lys.
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