Abstract

Mutation breeding gives better results for crop improvement through genetic manipulations when compared to other conventional breeding techniques. The present work focused in order to find out the effect of chemical mutagens; EMS and DES on Setaria italica in M1 generation. The seeds of Tenai, variety CO(Te)7 treated with different concentration of EMS and DES. The LD50 was observed at 30mM in EMS and 40mM in DES. Selection studies were conducted to improve the yield and to generate genetic variability in different quantitative traits such as days to first bloom, plant height, number of leaves, number of nodes, length and breadth of ear head, 1000 seed weight and yield per plant. The results revealed that, all the parameters were decreased with increasing concentration in both EMS and DES, while days to first bloom was increasing with increasing concentration. According to the result all the parameters studied shows a negative direction towards crop improvement in M1 generation because of the stress caused by mutagenic treatment.

Highlights

  • Setaria italica is a member of Paniceideace and is the very close relative of several major feed, fuel, bio energy grass

  • The parameters of M1 generation focuses upon the effectiveness and efficiency of the mutagens in representing the plant with more genetic damage with maximum mutations which is to be inherited to the generation

  • For ethyl methane sulphonate (EMS) and DES treatment hundred healthy seeds were presoaked in distilled water for 6 h and were treated with different concentration (10, 20, 30, 40 and 50 mmol) freshly prepared aqueous solution of ethyl methane sulphonate for 6 h and of another set 100 seeds were soaked in DES solution in different concentration of 10, 20, 30, 40 and 50 mmol S1) and same set of 100 seeds were soaked in distilled water and used as control

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Summary

Introduction

Setaria italica is a member of Paniceideace and is the very close relative of several major feed, fuel, bio energy grass. It is an annual grass grown for human food. It is the second most widely grown planted sp. Identifying, analyzing and selecting the essential mutations in a predictable manner is the main feature for using the plant for the exploitation in crop improvement and breeding programmes with the diverse spectrum of genetic variability [2] The present investigation in the variety Co (Te) in foxtail millet (Tenai) with chemical induced mutagenesis enables to create variety of gene frequency in the plant beeding techniques.

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