Abstract
Summary Dimethylnitrosamine (DMN) and diethylnitrosamine (DEN) were tested for the formation of mutagenic metabolites in vitro using liver microsomal preparations from mice or rats or in vivo in the host-mediated assay using mice or rats as hosts. The indicator organism for mutagenesis was strain G-46 of Salmonella typhimurium (mutations were scored as histidine reversions). In the host-mediated assay, the bacteria were injected into the peritoneal cavity. DMN gave approximately the same maximum reversion frequencies under host-mediated assay condition whether mice or rats were used as hosts. In comparison, the mouse liver microsome preparation was more than ten times more active than the rat liver microsome preparation. DEN showed almost no mutagenic effect in the host-mediated assay. DEN's mutagenic action was clearly shown in the mouse liver microsome system, although its activity was much lower than DMN in the same system.
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