Mutagen Formation after Heating of Mixtures of Guanidino Compounds, Amino Acids and Glucose.

Abstract

The effect of guanidino compounds other than creatin(in)e on mutagen formation was tested after heating with amino acid and glucose in a solution at 128°C for 2h. The mutagenicity of a heated mixture of creatine, glycine and glucose to Salmonella typhimurium TA98 with S9 mix was 630 per mg of heated material. When L-arginine was mixed with glycine plus glucose, 39 revertants of TA98 were induced per mg of heated material. Other guanidino compounds such as NG-methyl-L-arginine, 1-methylguanidine, aminoguanidine and guanidine also yielded mutagens, inducing 6.0-64 revertants per mg of heated material. When guanidino compounds were heated with L-phenylalanine and glucose, mixtures also yielded mutagenicity. The mutagenicity of a heated mixture of creatine, L-phenylalanine and glucose to TA98 with S9 mix was 160 per mg of heated material. L-Arginine, NG-methyl-L-arginine, aminoguanidine and guanidine gave 1.9-21 revertants per mg of heated material. With the addition of 1-methylguanidine, a very potent mutagenicity of 200 per mg of heated material was observed.