Abstract
ObjectiveTo detect Ha-Ras oncogene mutations in stromal fibroblasts in biopsies in the cervical transformation zone. MethodsEight samples were selected of fresh frozen tissue taken from patients diagnosed with intraepithelial neoplasia of the cervix, squamous type (NIC I, NIC II, NIC III), cancer in situ, and invasive squamous cell carcinoma, tissue in which only stroma was represented, with no evidence of residual tumor epithelial component; from each, the control cervical paraffin fixed tissue was counted which confirmed the presence of squamous cervical neoplastic process. The detection of codon 12 Ha-RAS mutations was carried out with non radioactive PCR-SSCP and restriction enzyme analysis. ResultsThe revealed mutation that altered a glycine into a valine in the codon 12 of the Ha-RAS oncogene was detected in only one sample (12.5%), NIC III. ConclusionsThe presence of molecular alteration, punctual mutation of the Ha-RAS oncogene in the stroma of one of the cases analyzed represents an important change and suggests an alternate route that could involve stromal genetic stability in the generation of intraepithelial neoplasia and in the progression of cervical cancer.
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