Abstract
The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas.To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors.Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. The tumor tissue was microdissected from paraffin embedded biopsies. The mutation was detected by a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Overall, 46% of samples had K-ras gene mutations. Mutation frequency was 80, 56, 50 and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. When compared with the rest, gallbladder tumors had a significantly lower frequency of the mutation. Median survival for biliary tract tumors was 6 months, compared with 65 months for gallbladder tumors (p <0.05).Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors.
Highlights
The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas
The mutation was detected by a combination of polymerase chain reaction
Gallbladder carcinoma had the lower frequency of K-ras mutation
Summary
The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas. Aim: To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors. Material and Methods: Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. Mutation frequency was [80, 56, 50] and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. Conclusions: Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors (Rev Méd Chile 2005; 133: 1434-40). (Key Words: Biliary tract neoplasms; Gallbladder neoplasms; Genes, ras; Pancreatic neoplasms). El objetivo de este trabajo fue determinar la frecuencia de mutación en el codón 12 del gen K-ras, mediante PCR-RFLP, en tumores pancreático-biliares en nuestra población y correlacionarla con las características clínicas y morfológicas de los tumores
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