Mustard seed-associated endophytes suppress Sclerotinia sclerotiorum causing Sclerotinia rot in mustard crop.

Abstract

Mustard-rapeseed cultivation is affected by Sclerotinia sclerotiorum resulting in loss of oil yield and degradation of crop quality. This study adopted an environment friendly biocontrol approach of screening mustard endophytes against the pathogen. Two bacterial isolates, Bacillus safensis (TS46 bac4) and Bacillus australimaris (SM2) showed potential biocontrol activity under both in vitro and in vivo conditions. Dual culture assay reported 90% inhibition of fungal growth. The bacterial cell free supernatant of isolate SM2 showed 52.89% inhibition and the other isolate TS46 bac4 showed 57.97% inhibition. The crude (10mg/ml) and purified (10mg/ml) metabolite extract of SM2 showed 100% and 97% inhibition respectively. Both crude (10mg/ml) and purified (7.5mg/ml) metabolite extract of TS46 bac4 exhibited 99% inhibition of the pathogen. Antifungal lipopeptides: surfactin, iturin and fengycin were identified in bacterial metabolite extract of the isolates. Both strains promoted healthy germination and prevented the formation of any disease symptoms in seedling. The selected Bacillus strains applied by spray method showed better results against fungal infection on mustard leaf and stem. Microscopic studies revealed degradation of fungal mycelial growth by both isolates. These findings support the employment of the bacterial strains as potential biocontrol agents to reduce the effects of S. sclerotiorum in mustard-rapeseed.