Mushroom Dietary Fiber from the Fruiting Body of Pleurotus tuber-regium: Fractionation and Structural Elucidation of Nondigestible Cell Wall Components

Abstract

The cell wall of mushroom fruiting body is constituted of nondigestible macromolecules that are a rich source of dietary fiber with biological functions that are beneficial to human health. The cell wall components of an edible mushroom fruiting body from Pleurotus tuber-regium (PTR) were fractionated, and their chemical structures were investigated by chemical, physicochemical, and microscopic analyses. The present results suggest that the cell wall of the PTR mushroom fruiting body contains four main fractions: an outer fraction of polysaccharide and protein complex, which can be extracted using boiling water; a cold alkali-soluble fraction of heteropolysaccharides associated with a small amount of proteins; a hot alkali-soluble fraction of hyper-branched glucans; and an alkali-insoluble fraction of glucan-chitin complex with a normalized relative percentage of 3.6:21.9:55.7:18.8. The anomeric linkage of all the glucans was revealed by infrared spectroscopy to be β type. The structure of the major mushroom fruiting body cell wall polysaccharide (the hot alkali-soluble one, FHA-I) was elucidated by the methylation analysis to be composed of →1)-Glcp-(4→ linkages as the backbone with a 52% degree of branching consisting of →1)-Glcp-(6→ linkages in the side chains, whereas some →1)-Glcp-(3→ linkages might exist in the backbone or side chains. Size exclusion chromatography coupled with multiangle laser light scattering analysis revealed that FHA-I had a molecular weight of 4.224 × 10(6) g/mol and a root-mean-square radius of 30.4 nm. Both scanning electron and atomic force microscopy further showed the highly branched microstructure of FHA-I when dispersed in an aqueous sodium dodecyl sulfate solution.